下调和上调
化学
伊诺斯
丹参
内皮干细胞
细胞生物学
真皮成纤维细胞
成纤维细胞
体外
生物化学
生物
医学
病理
一氧化氮合酶
酶
中医药
替代医学
基因
作者
Hong Meng,Mengmeng Zhao,Ru-Ya Yang,Xiaofeng Deng,Hongyan Zhang,Yeongmin Choi,In‐Sook An,Sungkwan An,Yinmao Dong,Yifan He,Li Li,Miaomiao Guo,Fan Yi
摘要
Salvianolic acid B (SAB) is one of the main active ingredients of Salvia Miltiorrhiza. It has significant skin anti-aging, whitening, and sun protection properties.The study aimed at studying the mechanism underlying the effect of salvianolic acid Bon collagen synthesis, which has good anti-aging efficacy and modulates microcirculation.This study employed available public databases, bioinformatics methodologies, and the inverse docking approach to explore the effectiveness of SAB in the regulating collagen synthesis, and then used an human dermal fibroblast (HDF)- Human dermal microvascular endothelial cell (HDMEC) in vitro model to validate the predicted mechanism of SAB in influencing collagen synthesis.The results showed that NO production in SAB-treated HDMEC-conditioned medium was increased compared to that in control media, and the same tendency was also observed for growth factor production. SAB also upregulated HDMEC cellular eNOS and VEGF. When SAB-treated HDMEC conditioned medium was transferred to HDFs, the expression of collagen I, collagen III, and elastin in HDFs was upregulated and MMP-1 was downregulated.The results show that SAB regulates collagen through the HDMEC-HDF pathway. Furthermore, the mechanisms might be closely related to the microcirculation factors NO and VEGF.
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