少突胶质细胞
视网膜
视网膜神经节细胞
转染
细胞生物学
生物
视神经
神经科学
神经节
视网膜
髓鞘
细胞外
中枢神经系统
细胞培养
生物化学
遗传学
作者
Trent A. Watkins,Anja R. Scholze
出处
期刊:CSH Protocols
[Cold Spring Harbor Laboratory]
日期:2014-10-01
卷期号:2014 (10): pdb.top070839-pdb.top070839
被引量:1
标识
DOI:10.1101/pdb.top070839
摘要
In this article, we introduce methods for generating rapidly myelinating cocultures with reaggregates of purified retinal ganglion cells and optic nerve oligodendrocyte precursor cells. This coculture system facilitates the study of complex central nervous system neuronal–glial interactions and myelination. It enables control of the extracellular environment and allows the use of transfected, virally infected, mutant, or knockout neurons and/or glial cell types. It is therefore possible to assess the role of various signaling pathways and genes in myelination and node of Ranvier formation.
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