Gingival crevicular fluid during experimental gingivitis: A review of immune and tissue regulation

This review examined how human experimental gingivitis (EG) affects immune responses and tissue changes in the fluid around the gums. Researchers analyzed studies published from 1960 to 2024, focusing on those that looked at immune responses and tissue changes in gum fluid before and after participants stopped brushing their teeth to induce EG. The review included 30 studies with 594 participants, exploring various health markers in gum fluid. When EG was induced, areas of the gums showed increased plaque and markers of inflammation, indicating an inflammatory response. Specifically, two inflammatory proteins (interleukin [IL]-1α and IL-1β) were found in greater expression, which aligns with what previous research has shown in gum disease. However, levels of certain proteins that help attract monocytes and macrophages decreased, suggesting changes in how these cells communicate during EG. Additionally, the levels of growth factors and anti-inflammatory substances did not change significantly. Factors such as age, stress, and diabetes also influenced the outcomes. These findings help us understand early immune responses in gum disease, which is crucial for developing prevention strategies and improving oral health overall.