色谱法
血迹
移液管
溶解
DNA提取
裂解缓冲液
多路复用
毛细管电泳
DNA
碱裂解
污渍
化学
聚合酶链反应
生物
分子生物学
染色
生物化学
遗传学
基因
物理化学
dna疫苗
质粒
作者
Michael Klintschar,Franz Neuhuber
摘要
A modified alkaline lysis protocol for extracting DNA from forensically relevant specimens is evaluated and compared with the chelex 100 method. For whole blood, bloodstains and sperm stains, both methods yielded comparable results after amplification for a pentameric STR locus (HumCD4). The main advantages of the new method are: only approximately ten minutes and two pipetting steps are necessary and the expenses for the extraction are extremely low as only NaOH, TrisHCl buffer and a single microcentrifuge tube are required. Alkaline lysis also proved to yield DNA suitable for typing longer STRs by using dye-labeled primers and capillary electrophoresis. These advantages should render this protocol especially interesting for high-throughput laboratories in combination with multiplex PCR and fluorescent dye technology, although the storability of the extracts proved to be problematic.
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