精子
野猪
男科
顶体
电容
化学
氧化应激
生物
生物化学
医学
作者
Ya-Qi Li,Yusha Wang,Tao An,Yawen Tang,Mei Shi,Wenyu Zhang,Mei Xue,Xianzhong Wang,Jiaojiao Zhang
标识
DOI:10.1016/j.ijbiomac.2023.128768
摘要
Boar sperm quality, as an important indicator of reproductive efficiency, directly affects the efficiency of livestock production. Here, this study was conducted to improve the boar sperm quality by using a non-thermal dielectric barrier discharge (DBD) plasma. Our results showed that DBD plasma exposure at 2.1 W for 15 s could improve boar sperm quality by increasing exon methylation level of adenosine monophosphate-activated protein kinase (AMPK) and thus improving the glycolytic flux, mitochondrial function, and antioxidant capacity without damaging the integrity of sperm DNA and acrosome. In addition, DBD plasma could rescue DNA methyltransferase inhibitor decitabine-caused low sperm quality through reducing the oxidative stress and mitochondrial damage. Therefore, the application of non-thermal plasma provides a new strategy for reducing sperm oxidative damage and improving sperm quality, which shows a great potential in assisted reproduction to solve the problem of male infertility.
科研通智能强力驱动
Strongly Powered by AbleSci AI