Objective To study the clinical value of injection of Xiaozhiling under ultrasonography guidance for ovarian cyst.Methods Under the ultrasonography guidance,the ovarian cyst was punctured by 18G PTC and cyst fluid was taken out completely.After cyst was irrigate clean with normal saline or Metronidazole,1/2 cyst-liquid Xiaozhiling was injected into cyst and took out 5 min later,then 1/8 cyst-liquid Xiaozhiling was injected again.Three months later,the cysts were measured again.Results All of 34 cases(28 patients) were effective,the effective rate was 100%.28 cases were cured,the cure rate was 82.4%.No patients had serious adverse reaction and side-effect.No patients relapsed in half year.Conclusions Injection of Xiaozhiling under ultrasonography guidance is safe and effective for treating ovarian cyst.