A new method for time-frequency analysis of a signal, which combines the time-order representation and the Wigner-Ville distribution (WVD) has been presented in this paper. The time-order representation based on short-term Fourier-Bessel (FB) expansion, decomposes a multicomponent signal into a number of monocomponent signals, and then the WVD technique is applied on each component of the composite signal to analyze its time-frequency distribution (TFD). Simulation results with real bat signal are included to illustrate the effectiveness of the proposed method.