生物
病毒学
滴答声
蓖麻硬蜱
载体(分子生物学)
肩胛硬蜱
蜱传脑炎病毒
病毒
脑炎
细胞培养
硬蜱
硬蜱科
遗传学
重组DNA
基因
作者
Daniel Růžek,Lesley Bell‐Sakyi,Jan Kopecký,Libor Grubhoffer
出处
期刊:Virus Research
[Elsevier]
日期:2008-10-01
卷期号:137 (1): 142-146
被引量:44
标识
DOI:10.1016/j.virusres.2008.05.013
摘要
We undertook a comparative study of the susceptibility of different tick cell lines to infection with the European subtype of tick-borne encephalitis virus (TBEV), prototype strain Neudoerfl. The growth of TBEV was investigated in lines derived from vector Ixodes ricinus L. ticks (IRE/CTVM18, 19, and 20), as well as non-vector ticks, namely Ixodes scapularis Say (IDE2), Boophilus microplus Canestrini (BME/CTVM2), Hyalomma anatolicum anatolicum Koch (HAE/CTVM9), Rhipicephalus appendiculatus Neumann (RA-257) and recently established and herein described lines from the argasid tick Ornithodoros moubata Murray (OME/CTVM21 and 22). All the tick cell lines tested were susceptible to infection by TBEV and the virus caused productive infection without any cytopathic effect. However, there was a clear difference between the TBEV growth in vector and non-vector cell lines, since I. ricinus cell lines produced 100-1000-fold higher virus yield than the non-vector cell lines. The lowest virus production was observed in O. moubata and R. appendiculatus cell lines.
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