Glycosylation of Acyclic and Cyclic Aglycone Substrates by Macrolide Glycosyltransferase DesVII/DesVIII: Analysis and Implications

Studies of the macrolide glycosyltransferase, DesVII/DesVIII, showed that when multiple hydroxyl groups are present, no regiospecificity of glycosylation is observed for the linear substrates. In contrast, when cyclic substrates with multiple glycosylation sites were tested, it displayed excellent regiospecificity in most cases. These results clearly indicate that this enzyme pair is a valuable tool for glycodiversification.