声动力疗法
二氧化钛
活性氧
内化
纳米颗粒
X射线光电子能谱
化学
胶质母细胞瘤
抗体
透射电子显微镜
细胞凋亡
癌症研究
纳米技术
材料科学
细胞
医学
化学工程
生物化学
免疫学
工程类
冶金
作者
George P. Lee,Alexander Willis,Sebastian Pernal,Abhijit H. Phakatkar,Tolou Shokuhfar,Vincent Blot,Herbert H. Engelhard
出处
期刊:Nanomedicine
日期:2021-03-01
卷期号:16 (7): 523-534
被引量:11
标识
DOI:10.2217/nnm-2020-0452
摘要
Aim: We present data on sonodynamic therapy (SDT) against glioblastoma cells utilizing titanium dioxide (TiO 2 ) nanoparticles conjugated to anti-EGFR antibody. Materials & methods: TiO 2 nanoparticles were bound to anti-EGFR antibody to form antibody-nanoparticle conjugates (ANCs), then characterized by x-ray photoelectron spectroscopy and transmission electron microscopy. Cells underwent ultrasound and assessment on viability, reactive oxygen species and apoptosis were performed. Results: X-ray photoelectron spectroscopy analysis revealed the formation of an ANC. Transmission electron microscopy showed internalization of the ANCs by glioblastoma cells. With SDT, cell viabilities were reduced in the presence of ANCs, reactive oxygen species production was formed, but minimal effect on apoptosis was seen. Conclusion: For the first time, an ANC can be used with SDT to kill glioblastoma cells.
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