STUDIES ON THE TECHNICS OF ENZYMATIC HYDROLYSIS OF PROTEIN IN HOLOTHURIAN INTESTINE

The technics of enzymatic hydrolysis of protein in Holothurian processing wastes-Holothurian intestine by using single enzyme and mixed enzymes are studied. The hydrolysis effect of the multi-step enaymatic hydrolysis using A.S1398 neutral proteinase, papain(papain: neutral proteinase=1:1), and Flavourzyme is the best. Through the orthogonal test, the optimum conditions for two-step enzymatic hydrolysis using Flavourzyme are determined to be as follow: enzyme added 150 U/g, enzymolysis time 1.5 h, temperature 55 ℃, pH 7.0, the degree of hydrolysis is 88.08 %. Then under the scientific condense, mix and pack to produce nutritious health oral liquid.