Determination of Ephedrine in Rhizoma Pinelliae,Rhizoma Typhonii Flagelliformis and their Processed Products by HPLC

Objective To determine the contents of ephedrin in Rhizoma Pinelliae,Rhizoma Typhonii Flagelliformis and their processed products by HPLC.Methods The diamensil C18 column methanol 0.08%triethylamine water solution(18∶82) as the mobile phase,flow rate was 1.0 ml/min,wavelength was 210 nm.Results Within the range of 0.8～2.4 μg of ephedrin,the linearity of this method was good(r=0.999 7).The average recovery of the different processed products was 97.22% with RSD of 1.72%.Conclusion The contents of ephedrin in Rhizoma Pinelliae and Rhizoma Typhonii Flagelliformis are apparently different,which can offer consideration for evaluation of quality of them.