重组DNA
小泡
大肠杆菌
化学
下游加工
产量(工程)
细胞生物学
细菌
生物化学
生物
膜
材料科学
基因
遗传学
冶金
作者
Tara A. Eastwood,Karen Baker,Bree R. Streather,Nyasha Allen,Lin Wang,Stanley W. Botchway,Ian R. Brown,Jennifer R. Hiscock,Christopher Lennon,Daniel P. Mulvihill
标识
DOI:10.1016/j.crmeth.2023.100396
摘要
We describe an innovative system that exports diverse recombinant proteins in membrane-bound vesicles from E. coli. These recombinant vesicles compartmentalize proteins within a micro-environment that enables production of otherwise challenging insoluble, toxic, or disulfide-bond containing proteins from bacteria. The release of vesicle-packaged proteins supports isolation from the culture and allows long-term storage of active protein. This technology results in high yields of vesicle-packaged, functional proteins for efficient downstream processing for a wide range of applications from discovery science to applied biotechnology and medicine.
科研通智能强力驱动
Strongly Powered by AbleSci AI