Poly(ethylene) glycol monomethyl ethers – an alternative to poly(ethylene) glycols in protein crystallization

Poly(ethylene) glycol monomethyl ethers (Peg-mmes) are a series of methyl substituted poly(ethylene) glycols that have been used with some success in the crystallization of a number of hydrophobic proteins. Crystallization of a lipase from Humicola lanuginosa complexed with the C12 substrate analogue from Peg-mme 5000, an endoglucanase 1 and a 59 kDa fragment of human topoisomerase IIalpha crystallized from Peg-mme are described. The use of Peg-mme for improving the quality of crystals previously grown from normal poly(ethylene) glycol 8000 is also described. We suggest that these modified Peg-mmes should be regularly used in screening for crystallization.