单克隆抗体
离子色谱法
化学
色谱法
离子交换
抗体
细胞
杂质
离子
生物化学
生物
有机化学
免疫学
作者
Anurag S. Rathore,Vishwanath Hebbi
出处
期刊:Springer eBooks
[Springer Nature]
日期:2022-01-01
卷期号:: 179-186
标识
DOI:10.1007/978-1-0716-1450-1_10
摘要
Ion Exchange Chromatography has been a critical unit operation for manufacturing of therapeutic antibodies. Cation and anion exchange chromatography are used extensively to remove process-related as well as product-related impurities to obtain the final product. In this chapter, we describe the methods for separating and purifying charge variants and aggregates for manufacturing of monoclonal antibodies. The methods related to removal of host cell impurities such as host cell DNA and host cell proteins are also described. With minimal modifications, the protocols described here can be employed to purify any monoclonal antibody.
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