Objective To approach the effective standardized management for cleaning and disinfection of endo-scope,in order to prevent medical infection by using endoscope examination,enhance the medical care and assure the medical safety.Methods The results of random check up the cleaned and disinfected endoscopes and their accessorics were reviewed that be ready by using NCX12—Ⅲ automatic ultrasonic nebulization endoscope cleaning and disinfecting machine(2% glutaric dialdehyde)to treat-artificial cleaning endoscope during Jan.2006 and Dec.2007.According to the check up methods,the same investingated samples were divided into group A(endoscope inner sampling)and group B(endoscope surface sampling).Results Fourty-seven pieces of endoscope were investigated and samples 94 picces were picked,each group 47 pieces.All endoscopes in group A were 100% qualification rate,but there were two samples of group B in excess of clony numbers,the sum qualification rate was 96%,with the gastroscope 95%,enteroscope 92%,and bronchfibroscope 100%.Conclusion The standardized management for cleaning and disinfection of endoscope is the most important key to assure disinfection quality of endoscopes.Both endoscope inner sampling and surface samoling methods are suggested on detection and check up the effect of disinfection of endoscope,to correctly valuate the cleaned and disinfected quality of endoscope.