Integrins and ion channels: molecular complexes and signaling.

1. Integrin Structure and Functional Relation with Ion Channels Annarosa Arcangeli and Andrea Becchetti Abstract Introduction Fundamentals of Integrin Structure Physiological and Pathological Implications: an Outline of Current Trends Conclusion 2. Introduction to Ion Channels Chiara Di Resta and Andrea Becchetti Abstract Introduction The Physiology of Ion Channels Ion Channel Types Involved in Integrin?Mediated Signaling Conclusion 3. Biochemical Methods to Study the Interactions Between Integrins and Ion Channels Olivia Crociani Abstract Introduction Yeast Two?Hybrid Screening Affinity?Based Screening: IP Assays Pull?Down Assay Photoaffinity Labeling Techniques for Studying Transient Protein?Protein Interaction Far Western Blot Analysis (Far WB) High?Throughput Protein?Protein Interaction Analysis, Followed by V alidation of Candidate Interactors through Different Experimental Approaches Past and Future Application of Biochemical Approaches to Characterize the Interactions between Integrins and Ion Channels 4. Optical Methods in the Study of Protein?Protein Interactions Alessio Masi, Riccardo Cicchi, Adolfo Carloni, Francesco Saverio Pavone and Annarosa Arcangeli Abstract Introduction Forster Resonance Energy Transfer: The Molecular Ruler Intensity Versus Lifetime: Two Ways to Measure FRET Total Internal Reflection Fluorescence Microscopy (TIRFM) and Imaging of Membrane Proteins Antibody?Based Versus Fusion Protein?Based FRET: Principles Antibody?Based Versus Fusion Protein?Based FRET: Advantages and Disadvantages Application of Optical Methods to the Study of Integrins and Ion Channels Conclusion 5. Integrins and Signal Transduction Sara Cabodi, Paola Di Stefano, Maria del Pilar Camacho Leal, Agata Tinnirello, Brigitte Bisaro, Virginia Morello, Laura Damiano, Simona Aramu, Daniele Repetto, Giusy Tornillo and Paola Defilippi Abstract Overview of Integrin Structure The SFK?Fak?p130Cas Signaling Conclusion 6. Physical andFunctional Interaction between Integrins and hERG1 Channels in Cancer Cells Serena Pillozzi and Annarosa Arcangeli Abstract Introduction hERG1 Channels in Cancer Cells Effects of Integrin Activation on hERG1 Channels Integrins and hERG1 Channels form a Macromolecular Complex Effects of hERG1 Activation on Integrin Function and Signaling Conclusion 7. Coordinated Regulation of Va scular Ca2+ and K+ Channels by Integrin Signaling Peichun Gui, Jun?Tzu Chao, Xin Wu, Yan Yang, George E. Davis and Michael J. Davis Abstract Introduction Regulation of L?Type Calcium Channels by Integrin Activation Regulation of Ca2+?Dependent Potassium Channels by Integrin Activation Conclusion: The Physiological Relevance of Coordinated Regulation of CaL and BK Channels by Integrins 8. Adhesion?Dependent Modulation of Macrophage K+ Channels Margaret Colden?Stanfield Abstract Introduction Inwardly Rectifying K+ (Kir) Currents Physiologic and Pathophysiologic Roles of Macrophage Kir Channels Delayed, Outwardly Rectifying K+ (Kdr) Currents Physiologic and Pathophysiologic Roles of Macrophage Kdr Channels Conclusion 9. Integrin Receptors and Ligand?Gated Channels Raffaella Morini and Andrea Becchetti Abstract Introduction The Functional Significance of Integrins in the Adult Brain Integrins and Nicotinic Acetylcholine Receptors: Not Only the Neuromuscular Junction Conclusion 10. Integrins and Ion Channels in Cell Migration: Implications for Neuronal Development, Wound Healing and Metastatic Spread Andrea Becchetti and Annarosa Arcangeli Abstract Introduction The Role of Integrins in Cell Migration The Role of Ion Channels and Crosstalk with Integrins in Cell Migration Ca2+ Signaling and the Axonal Growth Cone Ion Channels as Adhesion Molecules The Cellular Environment and the Metastatic Process Invasiveness of Glial Tumors and Ion Channels Conclusion Index