斜纹夜蛾
生物测定
昆虫
食草动物
生物
通才与专种
抗性(生态学)
植物
夜蛾
夜蛾科
生态学
幼虫
栖息地
重组DNA
生物化学
基因
作者
Stefan Mielke,Debora Gasperini
出处
期刊:Methods in molecular biology
日期:2020-01-01
卷期号:: 69-78
标识
DOI:10.1007/978-1-0716-0142-6_5
摘要
Jasmonates are essential engineers of plant defense responses against many pests, including herbivorous insects. Herbivory induces the production of jasmonic acid (JA) and its bioactive conjugate jasmonoyl-L-isoleucine (JA-Ile), which then triggers a large transcriptional reprogramming to promote plant acclimation. The contribution of the JA pathway, including its components and regulators, to defense responses against insect herbivory can be evaluated by conducting bioassays with a wide range of host plants and insect pests. Here, we describe a detailed and reproducible protocol for testing feeding behavior of the generalist herbivore Spodoptera littoralis on the model plant Arabidopsis thaliana and hence infer the contribution of JA-mediated plant defense responses to a chewing insect.
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