滚动圆复制
小RNA
G-四倍体
多重位移放大
复式(建筑)
生物传感器
适体
组合化学
电化学
生物物理学
电极
化学
流离失所(心理学)
纳米技术
核酸
DNA
材料科学
检出限
分子生物学
生物
聚合酶链反应
生物化学
色谱法
聚合酶
基因
物理化学
心理学
心理治疗师
DNA提取
作者
Xiaoqi Tang,Yang Wang,Lin Zhou,Wenqing Zhang,Sha Yang,Lianyu Yu,Shuang Zhao,Kai Chang,Ming Chen
标识
DOI:10.1007/s00604-020-4143-9
摘要
Emerging evidence suggests that exosomal microRNAs are potential biomarkers for the early diagnosis and prognostic assessment of tumor. Here, we design a strand displacement-initiated G-quadruplex/rolling circle amplification (RCA) strategy for highly specific and sensitive electrochemical sensing of exosomal microRNAs. In the presence of exosomal miRNA-21, a locked nucleic acid (LNA)-labeled toehold mediated strand displacement reaction (TMSDR) is initiated, releasing output P2 to trigger the subsequent RCA reaction by hybridizing with the C-rich circular template. Then the obtained G-rich RCA products can bind to the probe anchored on the surface of gold electrode and generate G-quadruplex conformations. Based on the TMSDR-triggered G-quadruplex/RCA strategy, the detection limit of this electrochemical biosensor is down to 2.75 fM. Moreover, our biosensor exhibits excellent repeatability, stability, and high consistency compared to RT-PCR for clinical detection. In conclusion, this assay is expected to provide a hopeful strategy for the early non-invasive diagnosis and prognostic estimation of cancer. Graphical abstract Schematic illustration of electrochemical sensing of exosomal microRNAs based on strand displacement-initiated G-quadruplex/rolling circle amplification (RCA) strategy.
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