Cloning of E Gene of PRRS Virus and Construction of Its Expressing Vectors

The E gene fragments of PRRS strain BJ-4 have been amplified and cloned into plasmid pBV221 and pPIC9K.Recombinant plasmid was constructed and transformed into the JM109.The result indicated that the recombinant vectors were successfully constructed.Positive clones of target genes were screened by PCR and identified with restriction endo-nuclease analysis.The positive clone of recombinant prokaryotic expression vector was induced and expressed with ELISA showed that it could react with the antibody to PRRSV.The results showed that E Protein of PRRSV was expressed in prokaryote system.The prokaryotic expression vector and eucaryotic expression vector were successfully constructed,which would be useful for production of gene vaccination in future.