Objective To explore the accuracy and influencing factors of ultrasonography in the prenatal screening for fetal cardiac malformations. Methods The clinical data of 1 200 pregnant women undergoing fetal cardiac ultrasound screening in our hospital were retrospectively analyzed. Four chamber views, left ventricular outflow tract, right ventricular outflow tract, and three vessels trachea view were used to screen the heart systematically. Results 25 cases were diagnosed as cardiac malformations prenatally, and 26 cases were conformed as cardiac malformations after delivery, with the diagnosis accuracy of 96.15%(25/26). Four chamber view prenatal plus beam method for detecting fetal heart 3 basic section(four chamber views, left and right ventricular outflow tract view.The display rate at 22~30 weeks gestation was up to 98%, at 31~42 weeks and 18~21 weeks gestation were 88.67%and 79.33%, respectively. Conclusion In prenatal ultrasound screening for fetal cardiac malformations, we should pay attention to choose the right section in pregnant women, appropriate gestational age in order to improve accuracy.