自愈水凝胶
明胶
组织工程
生物医学工程
材料科学
脂肪组织
透明质酸
伤口愈合
干细胞
新生血管
真皮
血管生成
体内
细胞生物学
化学
外科
病理
高分子化学
解剖
医学
癌症研究
生物
生物技术
生物化学
作者
Gözde Eke,Naşide Mangır,Nesrin Hasırcı,Sheila MacNeil,Vasıf Hasırcı
出处
期刊:Biomaterials
[Elsevier]
日期:2017-06-01
卷期号:129: 188-198
被引量:324
标识
DOI:10.1016/j.biomaterials.2017.03.021
摘要
The aim of this study was to design a dermal substitute containing adipose derived stem cells (ADSC) that can be used to improve the regeneration of skin on difficult wound beds by stimulating rapid neovascularization. This was achieved by first synthesizing methacrylated gelatin (GelMA) and methacrylated hyaluronic acid (HAMA) precursors which could be stored at -80 oC after lyophilisation. Polymer precursors were then dissolved in media (in 15:1 ratio), ADSCs added together with the photoinitiator and crosslinked with 40 s of UV. Hydrogels degraded by 50% over 3 weeks in an in vitro environment. ADSC loaded hydrogels could be easily handled with forceps (compressive modulus was 6 kPa). Transparency of the gel would allow a full field-of-view of a wound site. The hydrogels provided a suitable microenvironment for ADSC proliferation as shown by the filopodia observed in confocal micrographs. In vivo studies demonstrated that stem cell loaded hydrogels increased vascularization by up to 3 fold compared to their cell free counterparts. In conclusion, GelMA/HAMA hydrogels loaded with ADSC showed the desired proliferative and angiogenic properties essential to promote angiogenesis for wound healing and improving survival of tissue engineered skin.
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