识别序列
限制性酶
核酸内切酶
质粒
劈理(地质)
DNA
生物
分子生物学
序列(生物学)
限制性片段
碱基对
遗传学
断裂(地质)
古生物学
作者
Richard D. Morgan,C. Calvet,Marc A. Demeter,R Agra,Huimin Kong
摘要
N.BstNBI is a unique restriction endonuclease isolated from Bacillus stearothermophilus. We have characterized the recognition sequence and the cleavage site of N.BstNBI. Mapping of cleavage sites of N.BstNBI showed that it recognizes an asymmetric sequence, 5' GAGTC 3', and cleaves only on the top strand 4 base pairs away from its recognition sequence. To verify the nicking activity of N. BstNBI, we have constructed two plasmids containing a single recognition sequence (pNB1) or no recognition site (pNB0). When pNB1 and pNB0 were incubated with the enzyme, N.BstNBI nicked only the plasmid pNB1, suggesting that N.BstNBI is a specific nicking endonuclease.
科研通智能强力驱动
Strongly Powered by AbleSci AI