预言酚氧化酶
生物
舍宾
蛋白酵素
棉铃虫
抗菌肽
丝氨酸蛋白酶
毒液
蛋白酶
微生物学
免疫系统
肽
生物化学
先天免疫系统
植物
酶
免疫学
幼虫
基因
作者
Lei Zhou,Ruijuan Wang,Liu Zhe,Suke Shi,Caihua Chen,Haobo Jiang,Zhen Zou,Zhiqiang Lu
标识
DOI:10.1016/j.ibmb.2022.103895
摘要
Endoparasitoid wasps inject venom proteins into the hemocoel of host insects to ensure survival, growth, and development of their progenies by blocking host immunity. We previously identified ten serine protease inhibitors of the serpin superfamily in venom of the endoparasitoid wasp, Microplitis mediator, but it is unclear how these inhibitors may interact with host immune serine proteases. In this study, we investigated the functions of two serpins, MmvSPN-1 and MmvSPN-2, in the regulation of humoral immune responses in two hosts, the oriental armyworm Pseudaletia separate and the cotton bollworm Helicoverpa armigera, by dsRNA knockdown and biochemical assays using recombinant proteins. Knockdown of the two serpins resulted in increases in prophenoloxidase (PPO) activation and antimicrobial peptide (AMP) production in the hosts. After injection into the host hemocoel, the recombinant serpins inhibited PPO activation and AMP transcription. Mass spectrometry analysis of the pull-downs and in vitro reconstitution experiments revealed that HacSP29, a clip-domain serine protease in H. armigera, is the target of these two serpins. Therefore, these two inhibitors in the wasp venom may protect eggs from attacks by melanization and AMPs in the host insects.
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