聚吡咯
生物传感器
循环伏安法
安培法
核化学
拉曼光谱
介电谱
碳纳米管
材料科学
铂纳米粒子
玻璃碳
扫描电子显微镜
检出限
化学工程
葡萄糖氧化酶
纳米颗粒
纳米复合材料
固定化酶
电极
化学
电化学
纳米技术
色谱法
复合材料
物理化学
工程类
物理
光学
作者
Debasis Maity,R.T. Rajendra Kumar
标识
DOI:10.1016/j.bios.2019.02.001
摘要
A highly sensitive and selective glutamate biosensor using glutamate Oxidase (GlUtOx) immobilized platinum nanoparticle (PtNP) decorated multiwall carbon nanotube (MWCNTs)/polypyrrole (PPy) composite on glassy carbon electrodes (GC) is demonstrated. PtNP decorated MWCNTs (Pt-MWCNTs), PPy and Pt-MWCNTs/PPy composite were characterized by Field Emission Scanning Electron Microscope (FESEM), X-ray diffraction (XRD) and Raman analysis to confirm the formation of the nanocomposite. The glutamate Oxidase (GlUtOx) was immobilized on a GC/Pt-MWCNTs/PPy and characterized by the cyclic voltammetry (CV) and impedance spectroscopy (EIS) analysis. The fabricated L-glutamate biosensor exhibited high sensitivity (723.08 µA cm-2 mM-1) with less response time (3 s) with a detection limit of 0.88 µM. The dynamic range from 10 to 100 µM with a correlation coefficient (R2) of 0.985 was observed for the L-glutamate biosensor. The analytical recovery of added L-glutamate acid (50 and 100 μM) in human serum soup were 96.1% and 97.5% respectively. The enzyme immobilized GC/Pt-MWCNTs/PPy/GlUtOx bioelectrode lost 12.6% and 23.8% of its initial activity after 30 days when stored at - 20 °C and 4 °C respectively.
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