雅普1
骨骼肌
生物
免疫印迹
细胞生长
细胞生物学
心肌细胞
内科学
内分泌学
转录因子
遗传学
基因
医学
作者
Ming‐Liang He,Weibo Zhang,Shan Wang,Ling Ge,Xiukai Cao,Shanhe Wang,Zehu Yuan,Xiaoyang Lv,Tesfaye Getachew,Joram M. Mwacharo,Aynalem Haile,Wei Sun
标识
DOI:10.1080/10495398.2022.2112688
摘要
This study aimed to understand the expression level of YAP1 in the skeletal muscle of Hu sheep and to reveal the regulatory mechanism of YAP1 on Hu sheep skeletal muscle satellite cells (SMSCs). Previous research by our group has found that YAP1 may affect the growth and development of Hu sheep skeletal muscle. In the present study, we found the expression of YAP1 in the skeletal muscle is higher than in other tissues of Hu sheep. Then, we detected the effect of YAP1 on proliferation and differentiation in Hu sheep SMSCs. According to the results of qPCR, CCK-8, EDU, and Western blot, compared to the group of negative control, overexpression of YAP1 promoted the proliferation and inhibited the differentiation of SMSCs according to the results of qPCR, CCK-8, EDU, Western blot, while the interference of YAP1 was on the contrary. Overall, our study suggests that YAP1 is an important functional molecule in the growth and development of skeletal muscle by regulating the proliferation and differentiation of SMSCs. These findings are of great use for understanding the roles of YAP1 in the skeletal muscle of Hu sheep.
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