The Research on Cellulase Production of Green Liquor Pretreated Corn Stover

The effect of green liquid pretreated corn stover on cellulase production of Trichoderma reesei was investigated.Inspecting the influence of pretreatment conditions on cellulase production of corn stover at different cooking temperature,total alkali charge and sulfidity by orthogonal experiment,we could conclude from range and variance analysis that the extent of impact to cellucase production of green liquor pretreated corn stover was in turn total alkali charge,cooking temperature and sulfidity.The optimum filter paper activity(FPA) reached 2.6 IU/mL.FPA productions from corn stover pretreated by cooking temperature 140 ℃ and 170 ℃,total alkali charge 4 %,sulfidity 0,20 %,30 % and 40 %,were comprehensively compared and evaluated.It was found that the optimum conditions,i.e.,cooking temperature 140 ℃,total alkali charge 4 %,sulfidity 0,might ensure the value of FPA on and avoid the loss of materials.The results showed that green liquid pretreatment for corn stover was feasible for cellulase production of Trichoderma reesei.Taking corn stover pretreated by cooking temperature 140 ℃,total alkali charge 4% and sulfidity 0 as carbon sources whose concentration was 12 g/L,FPA and β-glucosidase activity(β-GA) were up to 2.5 IU/mL and 1.3 IU/mL respectively.The enzyme production period was 5 days.