Optimization of Enzymatic Extraction Technology of Polysaccharides from Seaweed by Response Surface Methodology

Objective:To establish optimal extraction conditions of polysaeeharides from Seaweed treated with cellulose.Methods: Seaweed polysaccharides content was determined by the phenol-sulfuric acid method and the yield of seaweed polysaccharides was the induce to optimize extraction process by a combination of response surface methodology(RSM).The cellulase concentration,enzymolysis temperature and enzymolysis time were selected as factors to study the influence of the extraction process.Results: Polysaccharide yield was most significantly affected by enzymolysis temperature,followed by extraction time,cellulase concentration.The optimal conditions of variables were determined as follows: cellulase concentration 1.2%,enzymolysis temperature 46.5 ℃,and enzymolysis time 100 min.Under the conditions above,the yield of polysaecharides was 4.64%.Conclusion:Compared with the extraction by water,this method can increase 7.41% in polysaccharide yield with less cost and higher efficiency.