IL-25 induces expression of Th2 inflammatory factor by nuocyte in asthma of mouse

Objective To study the effect of Interleukin-25 on expressing Th2 inflammatory factor in mouse asthma by nuocyte cells. Methods Nuocytes were collected from mouse spleen of OVA-induced asthma model by density gradient centrifugation. Samples were divided into 3 groups according to different intervention conditions:Interleukin-25 group; control group; anti-Interleukin-25 group. The expression of Interleukin-5 and Interleukin-13 proteins in nuocyte cells supernatant was detected by ELISA. The expression of Interleukin-5 and Interleukin-13 in nuocyte cells was detected by Western blot. The expression of Interleukin-5 and Interleukin-13 mRNA was detected by RT-PCR. The number of nuocyte cells was detected by flow cytometry. Results The expression of Interleukin-5 and Interleukin-13 protein and related gene in Interleukin-25 group was higer than that in the control group or anti-Interleukin-25 group( P 0. 05). The number of nuocyte cells in Interleukin-25 groupwas more than that in control group or anti-interleukin-25( P 0. 05). Conclusions Interleukin-25 can promote the expressing Interleukin-5 and Interleukin-13 in asthma by nuocyte cells and promote the occurrence and development of asthma.