Effect of menadione and plumbagin on glutathione.

The GSH and GSSG levels were expressed in nmols/mg of cell. Normal Growth refers to a non-stress condition while menadione or plumbagin stressed refers to cells stressed with 20 mM menadione or 7.0 μM plumbagin for 1 h, respectively. Menadione and Plumbagin treated and stressed refers to cells pre-treated with 0.5 mM menadione or 0.5 μM plumbagin for 1 h before being stressed with the same drugs. The means obtained for drug treated cells were compared with the normal growth condition of each strain using a t-student test and * represents statistically different results at P<0.05. ND–not determined.