In situ growth of imine‐based covalent organic framework as stationary phase for open‐tubular capillary electrochromatographic separation

Designing advanced stationary phases to improve separation efficiency is essential in capillary electrochromatography. Due to their outstanding performance, covalent organic frameworks have recently demonstrated considerable promise in the field of separation science. Herein, an open‐tubular capillary electrochromatography method was reported using porous imine‐based covalent organic framework with sufficiently available interaction sites as stationary phase. The imine‐based covalent organic framework coated capillary was easily prepared via an in situ growth method at room temperature, and its separation performance was evaluated, indicating the high separation efficiency for three types of analytes, including herbicides, polybrominated dibenzofurans, and bisphenols. Moreover, the imine‐based covalent organic framework coated capillary showed good reproducibility and stability, with intraday ( n = 3), interday ( n = 3), and column‐to‐column ( n = 3) relative standard deviations of retention time and peak areas of less than 5%. The separation efficiency of the coated capillary remained unchanged even after 200 runs and the maximum theoretical plates reached up to 85 595 N/m for 4,4′‐ethylidenebisphenol. It was predicted that the imine‐based covalent organic framework stationary phase would be a strong contender for chromatographic separation with high efficiency.