原位
共焦
细胞计数
细胞
共焦显微镜
显微镜
管道(软件)
荧光
荧光显微镜
生物医学工程
病理
化学
计算机科学
生物
细胞生物学
医学
生物化学
光学
物理
有机化学
细胞周期
程序设计语言
作者
Ouzéna Bouadi,Tuan Leng Tay
出处
期刊:Methods in molecular biology
日期:2023-08-29
卷期号:: 505-518
标识
DOI:10.1007/978-1-0716-3437-0_33
摘要
Using the open-source image analysis software CellProfiler to automatically quantify antibody-stained or fluorescently labeled macrophages in situ provides accurate and reproducible cell counts. It is a vastly enhanced alternative method to both manual cell counting and estimation of cell marker expression based on fluorescence intensity. Quantification of tissue-resident macrophages acquired on widefield or confocal microscopes can be batch processed using our pipeline to produce data within minutes.
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