Comparison of three PCR methods in diagnosis of porcine pseudorabies

Three different fragments,262 bp, 217 bp lying PRV gD gene and the 1 203 bp integrated PRV gD gene were amplified by PCR using three pairs of primers for the diagnosis of pig pseudorabies. Through comparison of the three PCR methods,it was found that they provided excellent specificity and sensitivity to diagnosis of pseudorabies and the amplification of 262 bp fragment could be used for rapid diagnosis of PRV infection in pigs making annealing-elongation on the same temperature, taking less than l hour to complete.