脐静脉
辛迪康1
细胞粘附分子
细胞外基质
流式细胞术
人脐静脉内皮细胞
细胞生物学
动脉粥样硬化
生物
分子生物学
病理
化学
体外
细胞
医学
内科学
遗传学
作者
Katharina Urschel,Karsten P. Hug,Hanxiao Zuo,Michael Büttner,Roman Furtmair,Constanze Kuehn,Florian M. Stumpfe,Balázs Botos,Stephan Achenbach,Yan Yuan,Barbara Dietel,Miyuki Tauchi
标识
DOI:10.3390/ijms241411595
摘要
Retention of circulating lipoproteins by their interaction with extracellular matrix molecules has been suggested as an underlying mechanism for atherosclerosis. We investigated the role of glypican-4 (GPC4), a heparan sulfate (HS) proteoglycan, in the development of endothelial dysfunction and plaque progression; Expression of GPC4 and HS was investigated in human umbilical vein/artery endothelial cells (HUVECs/HUAECs) using flow cytometry, qPCR, and immunofluorescent staining. Leukocyte adhesion was determined in HUVECs in bifurcation chamber slides under dynamic flow. The association between the degree of inflammation and GPC4, HS, and syndecan-4 expressions was analyzed in human carotid plaques; GPC4 was expressed in HUVECs/HUAECs. In HUVECs, GPC4 protein expression was higher in laminar than in non-uniform shear stress regions after a 1-day or 10-day flow (p < 0.01 each). The HS expression was higher under laminar flow after a 1 day (p < 0.001). Monocytic THP-1 cell adhesion to HUVECs was facilitated by GPC4 knock-down (p < 0.001) without affecting adhesion molecule expression. GPC4 and HS expression was lower in more-inflamed than in less-inflamed plaque shoulders (p < 0.05, each), especially in vulnerable plaque sections; Reduced expression of GPC4 was associated with atherogenic conditions, suggesting the involvement of GPC4 in both early and advanced stages of atherosclerosis.
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