扫描电镜
斯托克斯位移
荧光
显微镜
化学
荧光显微镜
超分辨显微术
生物物理学
受激发射
光化学
纳米技术
材料科学
光学
激光器
物理
生物
作者
Ilya Likhotkin,Richard Lincoln,Mariano L. Bossi,Alexey N. Butkevich,Stefan W. Hell
标识
DOI:10.1101/2022.12.02.518850
摘要
ABSTRACT We designed caging-group-free photoactivatable live-cell permeant dyes with red fluorescence emission and ∼100 nm Stokes shifts based on a 1-vinyl-10-silaxanthone imine core structure. The proposed fluorophores undergo byproduct-free one- and two-photon activation, are suitable for multicolor fluorescence microscopy in fixed and living cells and are compatible with super-resolution techniques such as STED (stimulated emission depletion) and PALM (photoactivated localization microscopy). Use of photoactivatable labels for strain-promoted azide-alkyne cycloaddition and self-labeling protein tags (HaloTag, SNAP-tag), and duplexing of imaging channel with another large Stokes shift dye have been demonstrated.
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