Protective Effects of Z-ligustilide against Cytotoxicity Induced by Aβ_(25-35) in Neuron Cells

Objective To investigate the effects of Z-ligustilide(LIG) on Aβ25-35-induced cytotoxicity in human neuroblastoma SH-SY5Y cells.Methods Cells were incubated with 0.1,1.0,2.5,5.0 μg/mL LIG and then were exposed to 50 μmol/L Aβ25-35 to induce cytotoxicity in SH-SY5Y cell to establish the AD model in vitro.Cell viability was test by MTT method.Proand anti-apoptosis protein levels were investigated by Western blot.Results After exposure to Aβ25-35,the cell viability decreased significantly,and the expression of pro-apoptosis protein——Bax,cleaved caspase 3,cytochrome C,caspase 8 was up regulated while the anti-apoptosis protein——Bcl-2 was down regulated.However,the treatment of LIG(0.1,1.0,2.5,5.0 μg/mL) significantly restored these changes.Conclusion LIG has the protective effect against cytotoxicity induced by in SH-SY5Y cells via inhibit the apoptosis induced by Aβ25-35.