呼吸上皮
上皮
气道
细胞因子
免疫学
呼吸道
粘液
白细胞介素13
免疫系统
细胞生物学
生物
白细胞介素
医学
呼吸系统
病理
解剖
外科
生态学
作者
Jamie L. Everman,Cydney Rios,Max A. Seibold
出处
期刊:Methods in molecular biology
日期:2018-01-01
卷期号:: 419-432
被引量:12
标识
DOI:10.1007/978-1-4939-7896-0_30
摘要
The airway epithelium lines the respiratory tract and provides the primary protective barrier against inhalational insults including toxic environmental substances and microorganisms. The airway epithelium also plays a critical role in regulating airway immune responses. The airway epithelial response to the type 2 cytokine, interleukin-13 (IL-13), is critical to airway inflammation, mucus production, and airway hyperresponsiveness present in asthma. Relevant primary cell models of the human airway epithelium are needed to investigate the biology of IL-13-mediated airway epithelial effects. Here, we describe the generation of a differentiated mucociliary human airway epithelium using an in vitro air–liquid interface (ALI) culture model system. We also describe methods to stimulate this culture model with IL-13 and harvest cells and biomolecules to interrogate cellular and molecular aspects of the airway epithelial IL-13 response.
科研通智能强力驱动
Strongly Powered by AbleSci AI