铜
膜
细胞毒性
金属
细菌
电化学
电解质
抗菌活性
纳米材料
阴极
化学
阳极
材料科学
纳米技术
核化学
冶金
电极
生物化学
体外
生物
物理化学
遗传学
作者
Yanfeng Shi,Yijun Li,Chao Huang,Yanfeng Xu,Yuanhong Xu
标识
DOI:10.1016/j.jcis.2022.12.094
摘要
Limited by the effective radius of metal ion release, higher concentrations of antibacterial agents are usually required to achieve satisfactory efficacy. Unfortunately, the potential cytotoxicity of metal ions limits the administered dose, which greatly hinders the widespread use of metal antibacterial agents. In this work, we used a convenient electrochemical method to prepare electropositive copper selenide (CuSe) nanosheets gathered from the cathode. Under physiological conditions, trace amounts of electrolytic CuSe (E-CuSe, 1 μg mL-1) could electrostatically bind to bacterial membranes and almost completely kill three resistant bacteria models (106 colony forming unit (CFU) mL-1). The extremely low effective dose of E-CuSe reaches a new benchmark in comparison with copper-based nanomaterials in other related studies. In addition, due to the reasonable coupling of selenium and copper, the as-prepared E-CuSe nanosheets exhibit lower cytotoxicity compared to copper oxide. As expected, the E-CuSe performed well in resistant bacteria-infected wound healing in rats, rapidly promoting wound tissue with a diameter of about 1 cm recovery within 7 days. Transcriptome analysis revealed the E-CuSe mainly acted on the membrane transport and DNA synthesis systems of bacterial cells. This work presents an efficient and in-depth paradigm for the scientific design and inactivation mechanism of metal antibacterial agents.
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