适体
化学
多路复用
四环素
抗生素
四环素类抗生素
胶体金
检出限
土霉素
组合化学
色谱法
氯霉素
纳米技术
纳米颗粒
生物化学
材料科学
分子生物学
生物
生物信息学
作者
Yifan Wu,Pengcheng Huang,Fang Wu
出处
期刊:Food Chemistry
[Elsevier]
日期:2020-01-01
卷期号:304: 125377-125377
被引量:108
标识
DOI:10.1016/j.foodchem.2019.125377
摘要
We devise a novel colorimetric aptasensor for multiplex antibiotics based on an ss-DNA fragment coordinately controlling gold nanoparticles (AuNPs) aggregation. The multifunctional aptamer (Apt) was elaborately designed to be adsorbed on AuNPs surfaces acting as a binding element for antibiotics and a molecular switch. Chloramphenicol (CAP) and tetracycline (TET) were selected as the model antibiotics. When one kind of antibiotics was added, the specifically recognized fragment of Apt can bind to it and dissociated, and the non-specific one coordinately controls AuNPs aggregation under high-salt conditions. Hence, different color changes of AuNPs solution can be used as the signal readout. The aptasensor exhibited remarkable selectivity and sensitivity for separate detection of TET and CAP, and the detection limits are estimated to be 32.9 and 7.0 nM, respectively. The analysis with the absorption spectroscopy and the smartphone are applied to detect antibiotics in real samples with consistent results and desirable recoveries.
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