软骨
阿格里坎
细胞外基质
细胞生物学
软骨细胞
球体
类有机物
解剖
基质(化学分析)
化学
蛋白多糖
生物
病理
关节软骨
骨关节炎
医学
体外
生物化学
替代医学
色谱法
作者
Shuichi Mizuno,Eiichiro Takada,Naomi Fukai
出处
期刊:Cells Tissues Organs
[S. Karger AG]
日期:2016-01-01
卷期号:202 (5-6): 382-392
被引量:6
摘要
Articular cartilage has multiple histologically distinct longitudinal depth zones. Development and pathogenesis occur throughout these zones. Cartilage explants, monolayer cell culture and reconstituted 3-dimensional cell constructs have been used for investigating mechanisms of pathophysiology in articular cartilage. Such models have been insufficient to reproduce zone-dependent cellular characteristics and extracellular matrix (ECM) upon investigation into cartilage development and pathogenesis. Therefore, we defined a chondrocyte spheroid model consistently formed with isolated chondrocytes from longitudinal depth zones without extrinsic materials. This spheroid showed zone-dependent characteristics of size, cartilage-specific ECM (collagen types I and II, aggrecan and keratan sulfate) and gene expressions of anabolic and catabolic molecules (matrix molecules and matrix metalloproteinase-13). In addition, the spheroid model is small enough to maintain the viability of cells and point symmetry to analyze the gradient of diffusive molecules. This spheroid organoid model will be useful to elucidate the mechanism of histogenesis and pathogenesis in articular cartilage.
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