Two New Chemical Constituents from Leaves of Perilla frutescens var. acuta

that is widely distributed throughout Korea, China, and Japan. In Korea, its leaves are used as a garnish and colorant for foods and as a traditional medicine for the treatment of asthma, cough, sore throat, dyspersia, insomnia, and diabetes.1,2 The EtOH extract of P. frutescens var. acuta was reported to have anti-allergic and anti-bacterial effects.3,4 Terpenoids, and phenolic compounds were also reported.5-7 In our continuing search for bioactive secondary metabolites from Korean medicinal plants, we investigated the constitu-ents of the leaves of P. frutescens var. acuta. Repeated column chromatographic separation of the MeOH extract led to the isolation of two new chemical constituents, includ-ing one phenolic compound, named perillascens (1) and one monoterpene glycoside, named perillaside (36), together with 34 known compounds (Figure 1). The structures of these new compounds were determined by spectroscopic methods, including 1D and 2D NMR (COSY, HMQC, and HMBC), HR-FAB-MS, optical rotation, and chemical reac-tion. Compound 1 was isolated as a colorless gum with positive optical rotation ( + 1.4). The molecular formula of 1 was determined to be C19H21NO5 on the basis of a [M + H]