后代
哺乳期
生物
DNA甲基化
内分泌学
内科学
表观遗传学
妊娠期
甲基化
脂质代谢
基因
怀孕
基因表达
遗传学
医学
作者
Huanling Yu,Shan Dong,Lifang Gao,Li Li,Yuan Xi,Wei Ma,Linhong Yuan,Rong Xiao
标识
DOI:10.1017/s0007114515000252
摘要
An epigenetic mechanism has been suggested to explain the effects of the maternal diet on the development of disease in offspring. The present study aimed to observe the effects of a maternal high-lipid, high-energy (HLE) diet on the DNA methylation pattern of male offspring in mice. Female C57BL/6J mice were fed an HLE diet during gestation and lactation. The genomic DNA methylations at promoter sites of genes in the liver, mRNA and protein levels of selected genes related to lipid and glucose metabolism were measured by microarray, real-time PCR and Western blot. The results indicated that the percentage of methylated DNA in offspring from dams that were fed an HLE diet was significantly higher than that from dams that were fed a chow diet, and most of these genes were hypermethylated in promoter regions. The nuclear protein content and mRNA levels of hypermethylated genes, such as PPARγ and liver X receptor α ( LXRα ), were decreased significantly in offspring in the HLE group. The results suggested that the DNA methylation profile in adult offspring livers was changed by the maternal HLE diet during gestation and lactation.
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