葡萄酒
电喷雾电离
质谱法
泰勒瓦
化学
色谱法
食品科学
作者
Keyuan Pu,Yue Wang,Hsing-Ju Wei,Jun Hu,Jiamin Qiu,Siyu Chen,Qian Liu,Yan Lin,Kwan‐Ming Ng
摘要
The commercial value of red wine is strongly linked to its geographical origin. Given the large global market, there is great demand for high-throughput screening methods to authenticate the geographical source of red wine. However, only limited techniques have been established up to now.Herein, a sensitive and robust method, namely probe electrospray ionization mass spectrometry (μ-PESI-MS), was established to achieve rapid analysis at approximately 1.2 min per sample without any pretreatment. A scotch near the needle tip provides a fixed micro-volume for each analysis to achieve satisfactory ion signal reproducibility (RSD < 26.7%). In combination with a machine learning algorithm, 16 characteristic ions were discovered from thousands of detected ions and were utilized for differentiating red wine origin. Among them, the relative abundances of two characteristic metabolites (trigonelline and proline) correlated with geographical conditions (sun exposure and water stress) were identified, providing the rationale for differentiation of the geographical origin.The proposed μ-PESI-MS-based method demonstrates a promising high-throughput determination capability in red wine traceability.
科研通智能强力驱动
Strongly Powered by AbleSci AI