中间灯丝
细胞生物学
细胞骨架
波形蛋白
微管
肌动蛋白
踏步
生物
蛋白质丝
微丝
细胞
生物化学
免疫学
免疫组织化学
作者
Juliane Behrens,Roxana Solga,Anja Ziemann,Raphael H. Rastetter,Carolin Berwanger,Harald Herrmann,Angelika A. Noegel,Christoph S. Clemen
标识
DOI:10.1016/j.ejcb.2016.04.004
摘要
Coronin 1C is an established modulator of actin cytoskeleton dynamics. It has been shown to be involved in protrusion formation, cell migration and invasion. Here, we report the generation of primary fibroblasts from coronin 1C knock-out mice in order to investigate the impact of the loss of coronin 1C on cellular structural organisation. We demonstrate that the lack of coronin 1C not only affects the actin system, but also the microtubule and the vimentin intermediate filament networks. In particular, we show that the knock-out cells exhibit a reduced proliferation rate, impaired cell migration and protrusion formation as well as an aberrant subcellular localisation and function of mitochondria. Moreover, we demonstrate that coronin 1C specifically interacts with the non-α-helical amino-terminal domain ("head") of vimentin. Our data suggest that coronin 1C acts as a cytoskeletal integrator of actin filaments, microtubules and intermediate filaments.
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