Distributed difference of intraepithelial lymphocyte and lamina propria lymphocyte of small intestine and large intestine in rat

Objective To study the distributed features of intraepithelial lymphocyte(IEL) and lamina propria lymphocyte(LPL) of small intestine(duodenum,jejunum,ileum) and large intestine(sigmoid colon) in rat.Methods Basilar membrane of bowel villi was marked by immunohistochemistry double staining,differentiate epithelia and lamina propria,and the amount of IEL and LPL were counted.Results The amount of IEL in various parts(duodenum,jejunum,ileum and sigmoid colon) of rat intestinal tract decreased in turn.On the contrary,the amount of LPL increased in turn.The difference among T cell subsets were obvious(All stated differences achieved statistical significance at P ≤0.05).Conclusion The method of double staining to account the number of IEL and LPL is very accurate,and IEL and LPL in different part of the intestine tract may play different biological roles in immune response of mucosa.