过氧化氢酶
超氧化物歧化酶
髓过氧化物酶
TBARS公司
溃疡性结肠炎
结肠炎
脂质体
硫代巴比妥酸
化学
抗氧化剂
炎症
药理学
生物化学
医学
免疫学
脂质过氧化
内科学
疾病
作者
Tareq Taha Jubeh,Mirela Nadler-Milbauer,Yechezkel Barenholz,Abraham Rubinstein
标识
DOI:10.1080/10611860600648429
摘要
Superoxide dismutase (SOD), 4-amino tempol (tempamine, denoted as TMN) and catalase were encapsulated into negatively charged liposomes. The activity of the antioxidants against dinitrobenzenesulfonic acid (DNBS) induced colitis was tested in the rat and compared to the anti-inflammatory activity of the native enzymes and free TMN. Inflammation severity was assessed by monitoring tissue myeloperoxidase (MPO) activity, thiobarbituric acid reactive species (TBARS) amounts and by comparing the weights of the dissected colons. In all cases, the liposomal preparations of the antioxidants were more effective than the free molecules in the treatment of the experimental colitis, probably due to the attachment of the negatively charged liposomes, and consequently a longer residence time and better uptake of the antioxidants to the inflamed mucosa. This study suggests that low and high molecular weight antioxidants delivered via anionic liposomes can serve as a novel targeted therapy to treat chronic inflammation of the colonic epithelium, such as ulcerative colitis.
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