Preparation of bowl-shaped polydopamine surface imprinted polymer composite adsorbent for specific separation of 2′-deoxyadenosine

Molecularly imprinted polymers (MIPs) have great potential as adsorbents for selective adsorption and separation of nucleoside compounds, but effectively enhancing the affinity of recognition sites by adjusting the forces between template molecules and functional monomers remains an important challenge. In this work, a surface imprinting strategy was used to construct bowl-shaped molecularly imprinted composite sorbents (BHPN@MIPs) based on polydopamine (PDA) particles and have achieved selective separation and purification of 2′-deoxyadenosine (dA). Where by the base complementary pairing interaction of the combined template molecule dA and the pyrimidine functional monomer can enhance the pre-assembly force, and the hydrophilic bowl-shaped PDA can provide a larger storage space contact efficiency of dA in the test solution, causing the site utilization much higher and improving the kinetic adsorption performance. The equilibrium adsorption time and maximum adsorption capacity of 60 min and 328.45 μmol·g−1 were observed by static adsorption experiments, and the selectivity experimental results showed an imprinting factor IF of 1.30. After four adsorption–desorption cycles, the initial adsorption equilibrium adsorption capacity of BHPN@MIPs still retained 91.14%. By evaluating the selective adsorption of dA in spiked human serum solutions, BHPN@MIPs can be used to selectively enrich and analyze target dA in complex biological samples.