相量
H&E染色
曙红
可视化
病理
染色
化学
荧光寿命成像显微镜
对比度(视觉)
人工智能
生物医学工程
荧光
计算机科学
光学
医学
物理
功率(物理)
电力系统
量子力学
作者
Teng Luo,Yuan Lu,Shaoxiong Liu,Dong Lin,Junle Qu
标识
DOI:10.1021/acs.analchem.7b01999
摘要
The phasor approach to fluorescence lifetime imaging microscopy (FLIM) is used to identify different types of tissues from hematoxylin and eosin (H&E) stained basal cell carcinoma (BCC) sections. The results suggest that working directly on the phasor space with the clustering assignment achieves immunofluorescence like simultaneous five or six-color imaging by using multiplexed fluorescence lifetimes of H&E. The phase approach is of particular effectiveness for enhanced visualization of the abnormal morphology of a suspected nidus. Moreover, the phasor approach to H&E FLIM data can determine the actual paths or the infiltrating trajectories of basophils and immune cells associated with the preneoplastic or neoplastic skin lesions. The integration of the phasor approach with routine histology proved its available value for skin cancer prevention and early detection. We therefore believe that the phasor analysis of H&E tissue sections is an enhanced visualization tool with the potential to simplify the preparation process of special staining and serve as color contrast aided imaging in clinical pathological examination.
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