Differential roles of microtubules in the two formation stages of membrane nanotubes between human mesenchymal stem cells and neonatal mouse cardiomyocytes

诺可达唑 间充质干细胞 微管 细胞生物学 细胞骨架 化学 流式细胞术 染色 分子生物学 生物 细胞 生物化学 遗传学
作者
Jianghui Zhang,Jing Zhang,Limin Zhao,Xin Yi,Sa Liu,Wei Cui
出处
期刊:Biochemical and Biophysical Research Communications [Elsevier BV]
卷期号:512 (3): 441-447 被引量:14
标识
DOI:10.1016/j.bbrc.2019.03.075
摘要

Membrane nanotubes (MNTs) are a kind of novel way for communication between two distant cells. It was recently shown that MNTs can be formed between distressed cardiomyocytes (CMs) and mesenchymal stem cells (MSCs). As a cytoskeleton-containing structure, the role of microtubules in MNTs is not fully understood. Here, we investigated this question. By membrane dye staining, we found that the numbers of MNTs between human MSCs (hMSCs) and distressed neonatal mouse CMs (NMCMs) increased gradually from 3 to 16 h and remained constant from 16 to 30 h, which were identified as active formation stage (the 1st stage, ≤16 h in coculture), and mature and stable stage (the 2nd stage, >16 h in coculture), respectively. In the 1st stage, more MNTs originated from hMSCs, whereas more MNTs originated from NMCMs in the 2nd stage. The formation of MNTs was affected when microtubules were disrupted by nocodazole in the 1st stage, but not in the 2nd stage. MNTs became shorter and thinner when microtubules were disrupted in the 2nd stage. Immunofluorescence staining and flow cytometry showed that mitochondria in hMSCs were transported into distressed NMCMs, which was suppressed by nocodazole in the 2nd stage. Tunnel staining showed that hypoxia/reoxygenation-induced apoptosis of NMCMs only in the 2nd stage could be rescued by direct, but not indirect, coculture with hMSCs. This rescue function was weakened when the mitochondrial functions of cocultured hMSCs were disrupted by EtBr or microtubules in cocultures were disrupted by nocodazole. All these results suggested that there are two stages for MNT formation, and microtubules played differential roles in the two stages: During the 1st stage, microtubules were required for MNT formation, whereas during the 2nd stage, microtubules were related to the morphological features of MNTs and played a key role in anti-apoptosis of MNTs by mitochondrial transfer.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
科目三应助summer采纳,获得10
1秒前
达乐发布了新的文献求助10
2秒前
wmz发布了新的文献求助10
5秒前
Winter发布了新的文献求助30
12秒前
gty发布了新的文献求助10
13秒前
15秒前
1206完成签到,获得积分10
16秒前
星星发布了新的文献求助10
18秒前
hnlgdx发布了新的文献求助10
20秒前
26秒前
李爱国应助wyk采纳,获得10
27秒前
一直向前发布了新的文献求助10
27秒前
27秒前
28秒前
xixi完成签到,获得积分10
28秒前
29秒前
30秒前
我是老大应助一直向前采纳,获得10
31秒前
xixi发布了新的文献求助10
32秒前
嗯对我呀完成签到,获得积分20
32秒前
34秒前
zg发布了新的文献求助10
35秒前
Zoeforever发布了新的文献求助10
35秒前
传奇3应助Gzq采纳,获得10
36秒前
SAL发布了新的文献求助10
40秒前
酷波er应助沉静的幻翠采纳,获得30
41秒前
44秒前
科研通AI2S应助害怕的慕晴采纳,获得10
45秒前
46秒前
达乐发布了新的文献求助10
48秒前
50秒前
gty发布了新的文献求助10
51秒前
yoyo完成签到 ,获得积分10
54秒前
大气睫毛完成签到,获得积分10
54秒前
hgjgjghgg发布了新的文献求助10
55秒前
五梁液发布了新的文献求助30
55秒前
Yuejun应助欣喜聪健采纳,获得50
55秒前
cquank完成签到,获得积分10
59秒前
wackykao完成签到 ,获得积分10
59秒前
全或无完成签到,获得积分10
59秒前
高分求助中
(禁止应助)【重要!!请各位详细阅读】【科研通的精品贴汇总】 10000
Robot-supported joining of reinforcement textiles with one-sided sewing heads 800
水稻光合CO2浓缩机制的创建及其作用研究 500
Logical form: From GB to Minimalism 500
2025-2030年中国消毒剂行业市场分析及发展前景预测报告 500
The Netter Collection of Medical Illustrations: Digestive System, Volume 9, Part III – Liver, Biliary Tract, and Pancreas, 3rd Edition 400
Elliptical Fiber Waveguides 400
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4171151
求助须知:如何正确求助?哪些是违规求助? 3706664
关于积分的说明 11695162
捐赠科研通 3392474
什么是DOI,文献DOI怎么找? 1860702
邀请新用户注册赠送积分活动 920531
科研通“疑难数据库(出版商)”最低求助积分说明 832740