Conductive Antifouling Sensing Coating: A Bionic Design Inspired by Natural Cell Membrane

Constructing antifouling coatings for biosensing interfaces is a major hurdle in driving their practical application. Inspired by the excellent antifouling properties of natural cell membranes, a conductive biomimetic antifouling interface coating is proposed, which highly mimics the excellent antifouling properties of biofilms while overcoming the low conductivity defects of conventional coatings. Polyethylene glycol-Au gel is selected as the support structure and electron transfer layer, on which phospholipids and ampholytes are applied to construct a hydration layer for antifouling. The coating maintains promisingly low adsorption in biological matrices such as whole blood, serum, and urine, and has been utilized to construct multimodal clinical assay systems that provide favorable concordance with clinical results. Thus, this conductive bio-coating breaks the last barrier of biosensors toward practical applications and possesses extremely significant application value.